BEP 2000 Advance System

BEP 2000 Advance System


The BEP 2000 Advance® System* and Enzygnost® and Novagnost® Assays (ELISA) help to detect infectious diseases (IDD), such as HIV, hepatitis, borreliosis and herpes but also bacteria, fungi, worms and other parasites. It provides fully automated processing of microtitration plates (MTP). BEP 2000 Advance together with Enzygnost and Novagnost offer:


Product Description

The BEP 2000 Advance® System* and Enzygnost® and Novagnost® Assays (ELISA) help to detect infectious diseases (IDD), such as HIV, hepatitis, borreliosis and herpes but also bacteria, fungi, worms and other parasites. It provides fully automated processing of microtitration plates (MTP).

Features and benefits

Automated ELISA processing
The BEP 2000 Advance® System is a fully automated microtitration plate (MTP) analyzer, perfectly designed for low- to mid-volume labs or specialty ID labs. It consolidates the laboratory workflow on one instrument with its broad menu of Enzygnost®and Novagnost® assays.

Advance Analyzer meets the demands for a high level of automation. It runs MTPs fully automated from sample to results and features a broad menu of infectious disease tests for the detection of HIV, hepatitis, borreliosis, herpes but also bacteria, fungi, worms and other parasites.
The system can process up to 4 MTPs simultaneously plus one MTP by reloading and up to 100 patient samples at the same time.
The requirements for the next generation of MTP-systems are included as well as newly developed unique software features. The well known and proven BEP concepts for GMP/GLP (good manufacturing practice / good laboratory practice) are also integrated in the BEP 2000 Advance Analyzer.
Convenience from the beginning
Designed for integration into your laboratory workflow, the BEP 2000 Advance Analyzer offers a sample-in / result-out process with online connection to your host computer. The specially designed front cover allows easy access to the individual components.

The BEP 2000 Advance System provides the following features and benefits:

Real automation for microtiter plates (MTP)

  • Processes up to 4 MTPs in one run and allows reloading of 1 additional MTP
  • Performs all assay steps: from reagent pipetting, through incubation, washing, reading to result evaluation
  • Records positive and negative events through in process control
  • Provides photometric reading and evaluation of results
  • Monitors the system status (accreditation) to increase safety and reliability
  • Reports qualitative and quantitative results (including alpha method, standard curves and user defined curves)

Integrated primary tube handling

  • Loads up to 100 primary tubes onboard – allows for increase up to 200 primary tubes by using the reloading option
  • Provides sample pre-dilution with Enzygnost and Novagnost assays and sample distribution from primary tubes
  • Allows positive identification of all samples and reagents through barcode identification

Disposable conductive tips to avoid carryover

  • Offers the choice of 2 different sizes of disposable tips (300µL and 1100µL) for single and multishot dispensing
  • Avoids carryover with specific hardware and software components designed and developed solely for the BEP 2000 Advance System
  • Allows clot detection

Flexible design

  • Runs round and flat bottom plates and allows the adaption of basically all ELISA MTPs
  • Comes with 37°C incubators with built-in shaker to provide homogeneous and accurate results: meets the demand for 3rd party assays

User-friendly, convenient software

  • Keeps training requirements at a minimum: it’s easy to learn and easy to use
  • Offers multiple software functions during the run
  • Monitors system status
  • Defines new work lists
  • Optimizes your laboratory workflow
  • Combines up to 12 tests / plate frame (same assay procedure)
  • Schedules loading time to increase efficiency
  • Provides bidirectional interface to a LIS (ASTM specification)
  • Downloads and uploads work list or results from and to LIS
  • Reduces operator errors and increases safety


Number of plates: Up to 4 plates at once
Number of samples Max 100 tubes, up to max. φ 16 mm, length up to 200 mm
Number of reagents Up to 4 reagent bottles in different sizes to be placed in special racks for Siemens reagents
Pre-dilution positions Positions for up to 4 pre-dilution plates
Number of tips Up to 5 tip racks for 300 μl or 1100 μl disposable tips
Loading Continuous loading of plates, samples, reagents and tips
Spectral range 400 - 700 nm
Dynamic range -0.100 to 3.000 O.D.
Accuracy +/- 0.005 or 2.5%
Linearity 0 - 2.000 O.D. +/- 1%
Detection Photo diode
Reading time < 15 seconds
Read modes OD and Kinetic mode
Filters Up to 8 positions
Pipetting System
Pipettor Liquid pipettor for disposable tips
Liquid level detection Standard setting
Volumes min. / max. Small tips: 10–300 μL / large tips: 1000 μL
Accuracy <-15% at 25 μL (aqua)
<-5% at 100 μL (aqua)
Precision <5% CV at 25 μL
<2.5% CV at 100 μL
Features Tip detection, mixing, multi-dispensing mode
Capacity 4 independent chambers
Temperature range Minimum temperature = RT + 5 °C
Maximum temperature = 50 °C
Accuracy +/- 1 °C mean of plate
Uniformity +/- 0.7 °C across plate
Capacity Max. 3 positions wash buffer + 1 distilled water
Wash head 1 x 8 with verify option
Dispense volume 200 - 2500 μl/well
Precision +/- 5% CV at 300 μl
Residual volume < 2.5 μl in U-shaped bottom
< 4 μl in flat bottom
Fluid alarms Low reagent, waste full
Features speeds Sweeps, soak, purge, top & bottom wash, variable pump
Bar Code
Bar code identification of samples, reagents and test plates possible
Personal computer
Microsoft Windows® - compatible
Software features
Operating system Microsoft Windows® 95, Microsoft Windows® 2000 or Microsoft Windows® 7
Interfaces ASTM interface
Flexible ASCII import of worklist sample ID's
Time Scheduling Schedules 4 plates with user options
Multiple assays per plate Up to 12 assays per plate
Export options Flexible ASCII export and report
Qualitative User definable result classes
Quantitative Linear to quadratic regression, sigmoidal, and many more
Language support Multiple languages possible
QA analysis Mean, SD, CV, Standard Error and Levey-Jennings
Power Supply
Universal a.c. input 100 - 260 VAC, 47 - 63 Hz
Typically max. 500 VA
Main fuses 4 A T
W x D X H 114 cm x 156 cm x 100 cm
(Depth with waste bag and completely opened drawer)
130 kg
67 dB (distance 1 m)
Environmental conditions
This standard applies equipment designed to be safe at least under the following conditions:
indoor use
altitude up to 2000 m
temperature 5 °C to 40 °C
maximum relative humidity 80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40 °C
mains supply voltage fluctuations up to +/- 10% of the nominal voltage
applicable rated pollution degree
Operating conditions
15 °C to 25 °C